( Division) : calculate a ratio of parameters + ( Addition) : combine parameters together It is easier to use the ‘T’ button in the graph window to do this much quicker.įlowJo provides a suite of mathematical functions to assist your analysis, described briefly below. By pressing Preview, your mono-variate plot can be previewed. Select a Reference parameter and then enter Min and Max values for the range. Zoom: Use Scaling to restrict the range of your plots to identify minor fluctuations in your histogram plots. It is easier to use the ‘T’ button in the graph window to do this much quicker. Lin/Log Conversion: Change scale by selecting Linear or Log from the Scale drop-down menu. Insert the Reference parameter, and then choose to multiply or add each value by any number to move the data points towards or away from 0. You can choose to create a ratio parameter with either linear or logarithmic scaling.Īdd Gain or Offset: Adjust your data’s display arithmetically. This is commonly necessary for calcium flux experiments, in which the ratio of Indo-1 fluorescence in two different channels is related to the calcium concentration. Ratio: Division can be used to calculate a ratio of any two collected parameters. This will eliminate the need to recreate the parameter(s) when using the data in a different workspace. It is important to note that although newly derived parameters are not added to the original data file, they can become part of a new data file if you choose to Export your file(s). These parameters behave like parameters that were collected: you can display them, calculate statistics on them, etc. FlowJo allows you to add new parameters to your data.
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